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Analysis of Extracellular Vesicles in the Tumor Microenvironment

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The Tumor Microenvironment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1458))

Abstract

Extracellular vesicles (ECV) are membrane compartments shed from all types of cells in various physiological and pathological states. In recent years, ECV have gained an increasing interest from the scientific community for their role as an intercellular communicator that plays important roles in modifying the tumor microenvironment. Multiple techniques have been established to collect ECV from conditioned media of cell culture or physiological fluids. The gold standard methodology is differential centrifugation. Although alternative techniques exist to collect ECV, these techniques have not proven suitable as a substitution for the ultracentrifugation procedure.

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Acknowledgments

This work was supported by a Movember Foundation/Prostate Cancer Canada Discovery Grant # D-2014-1 for Dr. K. Al-Nedawi.

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Correspondence to Khalid Al-Nedawi .

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Al-Nedawi, K., Read, J. (2016). Analysis of Extracellular Vesicles in the Tumor Microenvironment. In: Ursini-Siegel, J., Beauchemin, N. (eds) The Tumor Microenvironment. Methods in Molecular Biology, vol 1458. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3801-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3801-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3799-8

  • Online ISBN: 978-1-4939-3801-8

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