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Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer

  • Jitka Y. Sagiv
  • Sandra Voels
  • Zvi Granot
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1458)

Abstract

Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.

Key words

Neutrophils Cancer Isolation Density Enrichment Flow cytometry Cytotoxicity 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-CanadaHebrew University Medical SchoolJerusalemIsrael

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