Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

  • Hidetoshi Mori
  • Robert D. Cardiff
Part of the Methods in Molecular Biology book series (MIMB, volume 1458)


Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.

Key words

Autofluorescence Immunofluorescence Immunohistochemistry Formalin-fixed paraffin-embedded tissues Zinc fixation 



This work was supported by NCI grants (R33 CA183654, R21 CA183660) and Department of Defense Grant BC132309. We thank the Borowsky laboratory in Center for Comparative Medicine, University of California, Davis, for supporting experiments.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Center of Comparative MedicineUniversity of CaliforniaDavisUSA

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