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Immuno-Gold Labeling of Drosophila Follicles for Transmission Electron Microscopy

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Oogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1457))

Abstract

Detecting the localization of cellular components using gold nanoparticles has come to offer tremendous advantages in cell biology, allowing for the high resolution imaging of the cellular organization at the subcellular level. This is further aided by the breakthroughs in the cryopreparation of samples, which focus at the retention of antigenicity in efforts to mirror the native state of the tissues and cells as closely as possible. Herein, we describe the methodology for immuno-gold labeling of Drosophila follicles, following preparation of the samples using the Tokuyasu method for ultracryosectioning.

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Acknowledgements

This work is supported by a BBSRC grant (BB/L006324/1) awarded to Dr. Ioannis P. Nezis.

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Authors and Affiliations

  1. School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

    Panagiotis Tsapras, Antonia P. Sagona & Ioannis P. Nezis

Authors
  1. Panagiotis Tsapras
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  2. Antonia P. Sagona
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  3. Ioannis P. Nezis
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Corresponding author

Correspondence to Ioannis P. Nezis .

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Editors and Affiliations

  1. School of Life Sciences, University of Warwick, Coventry, United Kingdom

    Ioannis P. Nezis

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Tsapras, P., Sagona, A.P., Nezis, I.P. (2016). Immuno-Gold Labeling of Drosophila Follicles for Transmission Electron Microscopy. In: Nezis, I. (eds) Oogenesis. Methods in Molecular Biology, vol 1457. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3795-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3795-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3793-6

  • Online ISBN: 978-1-4939-3795-0

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