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Oogenesis pp 269–274Cite as

Immunohistochemistry of Paraffin Sections from Mouse Ovaries

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1457))

Abstract

Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme–substrate–chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin’s Fixative.

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Acknowledgement

This work was supported by The Scientific Research Projects Coordination Unit of Akdeniz University (Project Number: 2009.02.0122.010).

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Authors and Affiliations

  1. Department of Histology and Embryology, Medical Faculty, Akdeniz University, Antalya, 07070, Turkey

    Gokhan Akkoyunlu & Filiz Tepekoy

Authors
  1. Gokhan Akkoyunlu
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  2. Filiz Tepekoy
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Corresponding author

Correspondence to Gokhan Akkoyunlu .

Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Warwick, Coventry, United Kingdom

    Ioannis P. Nezis

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Akkoyunlu, G., Tepekoy, F. (2016). Immunohistochemistry of Paraffin Sections from Mouse Ovaries. In: Nezis, I. (eds) Oogenesis. Methods in Molecular Biology, vol 1457. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3795-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3795-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3793-6

  • Online ISBN: 978-1-4939-3795-0

  • eBook Packages: Springer Protocols

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