Abstract
Ribosomal RNA genes are highly repetitive and therefore not annotated in genome assemblies. We did recently analyze the epigenetic and architectural regulation of murine ribosomal genes by the Transcription Termination Factor I and made use of genome-wide histone modification ChIP-seq data. This method paper describes how repetitive genomic regions can be integrated into custom genomic assemblies and be used with genome-wide profiling data.
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Acknowledgment
Work in our laboratory is funded by the German Research Community (DFG, grants within the SFB960)
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Schwartz, U., Längst, G. (2016). Bioinformatic Analysis of ChIP-seq Data on the Repetitive Ribosomal RNA Gene. In: Németh, A. (eds) The Nucleolus. Methods in Molecular Biology, vol 1455. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3792-9_17
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DOI: https://doi.org/10.1007/978-1-4939-3792-9_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3790-5
Online ISBN: 978-1-4939-3792-9
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