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Cilia pp 123-147 | Cite as

Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left–Right Organizer

  • Shiaulou Yuan
  • Martina BruecknerEmail author
Protocol
  • 1.6k Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 1454)

Abstract

Cilia play a key role in the determination of the left–right axis in vertebrates by generating and sensing flow of extraembryonic fluid at the left–right organizer (LRO). Perception of cilia-directed flow triggers a calcium signaling cascade which originates within the cilium itself and then is relayed into the surrounding mesendodermal tissue, thereby directing organ situs via the nodal pathway. Two types of cilia, motile and immotile, function simultaneously to coordinate and direct asymmetric intraciliary calcium signaling cues in the LRO. Here, we describe tools, reagents, and methodologies for the visualization and manipulation of both cilia types as well as intraciliary calcium signaling in the LRO of zebrafish.

Key words

Cilium Nodal cilia Kupffer’s vesicle GECI 

Notes

Acknowledgments

This work was funded by NIH grant 1R01HL125885-01A1 (to M.B.) and a Hartwell Foundation postdoctoral fellowship (to S.Y.).

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© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of PediatricsYale University School of MedicineNew HavenUSA
  2. 2.Department of GeneticsYale University School of MedicineNew HavenUSA

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