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Methods for Studying Movement of Molecules Within Cilia

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Book cover Cilia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1454))

Abstract

The assembly of cilia and eukaryotic flagella (interchangeable terms) requires the import of numerous proteins from the cell body into the growing organelle. Proteins move into and inside cilia by diffusion and by motor-based intraflagellar transport (IFT). Many aspects of ciliary protein transport such as the distribution of unloading sites and the frequency of transport can be analyzed using direct in vivo imaging of fluorescently tagged proteins. Here, we will describe how to use total internal reflection fluorescence microcopy (TIRFM) to analyze protein transport in the flagella of the unicellular alga Chlamydomonas reinhardtii, a widely used model for cilia and cilia-related disease.

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Acknowledgement

This work was supported by a grant from the National Institutes of Health (GM110413).

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Authors and Affiliations

  1. Department of Cellular Biology, University of Georgia, 635 Biological Science Building, 1000 Cedar Street, Athens, GA, 30602, USA

    Karl F. Lechtreck

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  1. Karl F. Lechtreck
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Correspondence to Karl F. Lechtreck .

Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA

    Peter Satir

  2. Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Søren Tvorup Christensen

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© 2016 Springer Science+Business Media New York

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Lechtreck, K.F. (2016). Methods for Studying Movement of Molecules Within Cilia. In: Satir, P., Christensen, S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3789-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3789-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3787-5

  • Online ISBN: 978-1-4939-3789-9

  • eBook Packages: Springer Protocols

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