Abstract
Hair follicle morphogenesis is first induced by epithelial–mesenchymal interactions in the developing embryo. In the hair follicle, various stem-cell populations are maintained in specialized niches to promote repetitive hair follicle-morphogenesis, which is observed in the variable lower region of the hair follicle as a postnatal hair cycle. In contrast, the genesis of most organs is induced only once during embryogenesis. We developed a novel bioengineering technique, the Organ Germ Method, that employs three-dimensional stem cell culture for regenerating various organs and reproducing embryonic organogenesis. In this chapter, we describe a protocol for hair follicle germ reconstitution using adult follicle-derived epithelial stem cells and dermal papilla cells with intracutaneous transplantation of the bioengineered hair-follicle organ germ. This protocol can be useful not only for the clinical study of hair regeneration but also for studies of stem cell biology and organogenesis.
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Tezuka, K., Toyoshima, Ke., Tsuji, T. (2016). Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ. In: Hoffman, R. (eds) Multipotent Stem Cells of the Hair Follicle. Methods in Molecular Biology, vol 1453. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3786-8_9
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DOI: https://doi.org/10.1007/978-1-4939-3786-8_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3784-4
Online ISBN: 978-1-4939-3786-8
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