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Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ

  • Katsunari Tezuka
  • Koh-ei Toyoshima
  • Takashi TsujiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)

Abstract

Hair follicle morphogenesis is first induced by epithelial–mesenchymal interactions in the developing embryo. In the hair follicle, various stem-cell populations are maintained in specialized niches to promote repetitive hair follicle-morphogenesis, which is observed in the variable lower region of the hair follicle as a postnatal hair cycle. In contrast, the genesis of most organs is induced only once during embryogenesis. We developed a novel bioengineering technique, the Organ Germ Method, that employs three-dimensional stem cell culture for regenerating various organs and reproducing embryonic organogenesis. In this chapter, we describe a protocol for hair follicle germ reconstitution using adult follicle-derived epithelial stem cells and dermal papilla cells with intracutaneous transplantation of the bioengineered hair-follicle organ germ. This protocol can be useful not only for the clinical study of hair regeneration but also for studies of stem cell biology and organogenesis.

Key words

Hair follicle Organ Germ Method Stem cells Epithelial–mesenchymal interaction 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Katsunari Tezuka
    • 1
    • 2
  • Koh-ei Toyoshima
    • 1
    • 2
    • 3
  • Takashi Tsuji
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Center for Developmental BiologyRIKENKobeJapan
  2. 2.Organ Technologies Inc.TokyoJapan
  3. 3.Department of Regenerative Medicine, Plastic and Reconstructive SurgeryKitasato University of MedicineSagamiharaJapan
  4. 4.Research Institute for Science and TechnologyTokyo University of ScienceNodaJapan

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