Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay

  • Ying Zheng
  • Jen-Chih Hsieh
  • Julia Escandon
  • George CotsarelisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)


The hair follicle (HF) is a dynamic structure readily accessible within the skin, and contains various pools of stem cells that have a broad regenerative potential during normal homeostasis and in response to injury. Recent discoveries demonstrating the multipotent capabilities of hair follicle stem cells and the easy access to skin tissue make the HF an attractive source for isolating stem cells and their subsequent application in tissue engineering and regenerative medicine. Here, we describe the isolation and purification of hair follicle bulge stem cells from mouse skin, and hair reconstitution assays that allows the functional analysis of multipotent stem cells.

Key words

Hair follicle stem cell Bulge Hair reconstitution assay Regeneration 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ying Zheng
    • 1
  • Jen-Chih Hsieh
    • 1
  • Julia Escandon
    • 2
  • George Cotsarelis
    • 1
    Email author
  1. 1.Department of DermatologyUniversity of Pennsylvania Medical CenterPhiladelphiaUSA
  2. 2.Department of Dermatology and Cutaneous SurgeryUniversity of Miami, Miller School of MedicineMiamiUSA

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