Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

  • Ruifeng Yang
  • Xiaowei XuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)


The hair follicle undergoes lifelong cycling and growth. Previous studies have been focused on epithelial stem cells in the hair follicles. Neural crest stem cells (NCSCs) are pluripotent cells that can persist in adult tissues. We have previously demonstrated that human NCSCs can be isolated from hair follicles. Here, we present a protocol to isolate NCSCs from human hair follicles based on their specific surface-marker expression of CD271/HNK1 or CD271/CD49D (alpha4 integrin). NCSCs can be expanded in the culture as neural spheres or attached cells.

Key words

Protocol Neural crest stem cells Hair follicle Bulge 



This work was supported by NIH grants R01AR054593 and R01AR054593-S1 to X. Xu.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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