Nestin-Based Reporter Transgenic Mouse Lines

  • John Mignone
  • Natalia Peunova
  • Grigori EnikolopovEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)


Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.

Key words

Nestin Hair-follicle-associated pluripotent (HAP) Stem cells Transgenic animals Reporter lines Immunocytochemistry Nestin-GFP 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • John Mignone
    • 1
  • Natalia Peunova
    • 1
  • Grigori Enikolopov
    • 1
    Email author
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA

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