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Multipotent Stem Cells of the Hair Follicle pp 137–144Cite as

Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1453))

Abstract

Isolated whisker follicles from nestin-driven green fluorescent protein (ND-GFP) mice, containing hair-associated pluripotent (HAP) stem cells, were histocultured in three dimensions on Gelfoam® for 3 weeks for subsequent transplantation to the spinal cord in order to heal an induced injury with the HAP stem cells. The hair shafts were removed from Gelfoam®-histocultured whisker follicles, and the remaining parts of the whisker follicles, containing GFP-nestin-expressing (HAP) stem cells, were transplanted into the injured spinal cord of nude mice, along with the Gelfoam®. After 90 days, the mice were sacrificed and the spinal cord injuries were observed to have healed. ND-GFP expression was intense at the healed area of the spinal cord, as observed by fluorescence microscopy, demonstrating that the HAP stem cells were involved in healing the spinal cord. The transplanted whisker follicles produced remarkably long hair shafts in the spinal cord over 90 days and curved and enclosed the spinal cord. This result changes our concept of hair growth, demonstrating it is not limited to the skin and that hair growth appears related to HAP stem cells as both increased in tandem on the spinal cord.

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Acknowledgements

This studies described in the present chapter were supported by the National Institute of Neurological Disorders and Stroke grant NS086217.

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Authors and Affiliations

  1. AntiCancer Inc., San Diego, CA, USA

    Wenluo Cao, Fang Liu, Yasuyuki Amoh & Robert M. Hoffman

  2. Department of Surgery, University of California, San Diego, San Diego, CA, USA

    Wenluo Cao, Yasuyuki Amoh & Robert M. Hoffman

  3. Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, China

    Wenluo Cao & Fang Liu

  4. Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan

    Yasuyuki Amoh

Authors
  1. Wenluo Cao
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  2. Fang Liu
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  3. Yasuyuki Amoh
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  4. Robert M. Hoffman
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Corresponding author

Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. University of California, AntiCancer, Inc., San Diego, California, USA

    Robert M Hoffman

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Cao, W., Liu, F., Amoh, Y., Hoffman, R.M. (2016). Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice. In: Hoffman, R. (eds) Multipotent Stem Cells of the Hair Follicle. Methods in Molecular Biology, vol 1453. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3786-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3786-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3784-4

  • Online ISBN: 978-1-4939-3786-8

  • eBook Packages: Springer Protocols

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