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Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination

  • Nicholas W. Rufaut
  • Allan J. Nixon
  • Rodney D. SinclairEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 1453)

Abstract

Common human balding or hair loss is driven by follicle miniaturization. Miniaturization is thought to be caused by a reduction in dermal papilla size. The molecular mechanisms that regulate papilla size are poorly understood, and their elucidation would benefit from a tractable experimental model. We have found that dermal papilla cells from sheep spontaneously aggregate in culture to form papilla-like structures. Here, we describe methods for microdissecting dermal papillae from wool follicles, for initiating and maintaining cultures of ovine papilla cells, and for using these cells in an in vitro assay to measure the effect of bioactive molecules on aggregate size.

Key words

Hair follicle Dermal papilla Morphogenesis Aggregation In vitro Sheep Ovine Wool Hair loss 

Notes

Acknowledgements

The development of these culture methods was supported by the New Zealand Foundation for Research, Science and Technology (C10X0403), the Australian Wool Innovation Ltd. Sheep GENOMICS program (SG318), and the AgResearch Research and Capability Fund (A12258 and A13595).

References

  1. 1.
    Rogers GE (2006) Biology of the wool follicle: an excursion into a unique tissue interaction system waiting to be re-discovered. Exp Dermatol 15(12):931–949CrossRefPubMedGoogle Scholar
  2. 2.
    Richardson GD, Arnott EC, Whitehouse CJ et al (2005) Plasticity of rodent and human hair follicle dermal cells: implications for cell therapy and tissue engineering. J Investig Dermatol Symp Proc 10(3):180–183CrossRefPubMedGoogle Scholar
  3. 3.
    Yang CC, Cotsarelis G (2010) Review of hair follicle dermal cells. J Dermatol Sci 57(1):2–11CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Inamatsu M, Tochio T, Makabe A et al (2006) Embryonic dermal condensation and adult dermal papilla induce hair follicles in adult glabrous epidermis through different mechanisms. Dev Growth Differ 48(2):73–86CrossRefPubMedGoogle Scholar
  5. 5.
    Mahjour SB, Ghaffarpasand F, Wang H (2012) Hair follicle regeneration in skin grafts: current concepts and future perspectives. Tissue Eng Part B Rev 18(1):15–23CrossRefPubMedGoogle Scholar
  6. 6.
    Ohyama M, Veraitch O (2013) Strategies to enhance epithelial-mesenchymal interactions for human hair follicle bioengineering. J Dermatol Sci 70:78–87. doi: 10.1016/j.jdermsci.2013.02.004 CrossRefPubMedGoogle Scholar
  7. 7.
    Horne KA, Jahoda CA, Oliver RF (1986) Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat. J Embryol Exp Morphol 97:111–124PubMedGoogle Scholar
  8. 8.
    Jahoda CA, Oliver RF (1984) Vibrissa dermal papilla cell aggregative behaviour in vivo and in vitro. J Embryol Exp Morphol 79:211–224PubMedGoogle Scholar
  9. 9.
    Rufaut NW, Nixon AJ, Goldthorpe NT et al (2013) An in vitro model for the morphogenesis of hair follicle dermal papillae. J Invest Dermatol 133:2085–2088. doi: 10.1038/jid.2013.132 CrossRefPubMedGoogle Scholar
  10. 10.
    Elliott K, Stephenson TJ, Messenger AG (1999) Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J Invest Dermatol 113(6):873–877CrossRefPubMedGoogle Scholar
  11. 11.
    Ibrahim L, Wright EA (1982) A quantitative study of hair growth using mouse and rat vibrissal follicles: I. Dermal papilla volume determines hair volume. J Embryol Exp Morphol 72:209–224PubMedGoogle Scholar
  12. 12.
    Jahoda CA (1998) Cellular and developmental aspects of androgenetic alopecia. Exp Dermatol 7(5):235–248PubMedGoogle Scholar
  13. 13.
    Whiting DA (2001) Possible mechanisms of miniaturization during androgenetic alopecia or pattern hair loss. J Am Acad Dermatol 45(3 Suppl):S81–S86CrossRefPubMedGoogle Scholar
  14. 14.
    Rufaut NW, Goldthorpe NT, Wildermoth JE, Wallace OA (2006) Myogenic differentiation of dermal papilla cells from bovine skin. J Cell Physiol 209(3):959–966CrossRefPubMedGoogle Scholar
  15. 15.
    Reynolds AJ, Jahoda CA (1991) Hair follicle stem cells? A distinct germinative epidermal cell population is activated in vitro by the presence of hair dermal papilla cells. J Cell Sci 99(Pt 2):373–385PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Nicholas W. Rufaut
    • 2
  • Allan J. Nixon
    • 2
    • 3
  • Rodney D. Sinclair
    • 1
    • 2
    Email author
  1. 1.Epworth Research InstituteEpworth HospitalMelbourneAustralia
  2. 2.Department of MedicineUniversity of MelbourneMelbourneAustralia
  3. 3.Histopathology DepartmentWaikato District Health BoardHamiltonNew Zealand

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