Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation

  • Kazuo KishiEmail author
  • Ruka Hayashi
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)


Isolated dermal cells possess the capacity to induce hair growth. The cells cannot be expanded while they retain the capacity for hair induction, and lose their potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis (skin-derived precursors [SKPs]) possess hair-inducing activity. These observations provide two possibilities for the determination of the capacity for hair induction: capacity is dependent on either identity as a dermal cell or on the process of sphere formation. We developed a method that demonstrates cultivated mesenchymal cells derived from dermis and lung tissue possess in vivo hair-inducing capacity via sphere formation.

Key words

Mesenchymal cells Sphere formation Skin-derived precursors (SKPs) 


  1. 1.
    Yang CC, Cotsarelis G (2010) Review of hair follicle dermal cells. J Dermatol Sci 57:2–11CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Jahoda CA, Horne KA, Oliver RF (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311:560–562CrossRefPubMedGoogle Scholar
  3. 3.
    Jahoda CA, Reynolds AJ, Oliver RF (1993) Induction of hair growth in ear wounds by cultured dermal papilla cells. J Invest Dermatol 101:584–590CrossRefPubMedGoogle Scholar
  4. 4.
    Kishi K, Katsube K, Satoh H et al (2005) The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure. Cell Transplant 14:709–714CrossRefPubMedGoogle Scholar
  5. 5.
    Young TH, Lee CY, Chiu HC et al (2008) Self-assembly of dermal papilla cells into inductive spheroidal microtissues on poly(ethylene-co-vinyl alcohol) membranes for hair follicle regeneration. Biomaterials 29:3521–3530CrossRefPubMedGoogle Scholar
  6. 6.
    Toma JG, Akhavan M, Fernandes KJ et al (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784CrossRefPubMedGoogle Scholar
  7. 7.
    Biernaskie J, Paris M, Morozova O et al (2009) SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell 5:610–623CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Li L, Mignone J, Yang M et al (2003) Nestin expression in hair follicle sheath progenitor cells. Proc Natl Acad Sci U S A 100:9958–9961CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Amoh Y, Li L, Yang M et al (2004) Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells. Proc Natl Acad Sci U S A 101:13291–13295CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Amoh Y, Li L, Katsuoka K et al (2005) Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc Natl Acad Sci U S A 102:5530–5534CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Shimizu R, Okabe K, Kubota Y et al (2011) Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells. Exp Dermatol 20(8):679–681CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive SurgeryKeio University, School of MedicineTokyoJapan

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