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Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation

  • Kazuo KishiEmail author
  • Ruka Hayashi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)

Abstract

Isolated dermal cells possess the capacity to induce hair growth. The cells cannot be expanded while they retain the capacity for hair induction, and lose their potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis (skin-derived precursors [SKPs]) possess hair-inducing activity. These observations provide two possibilities for the determination of the capacity for hair induction: capacity is dependent on either identity as a dermal cell or on the process of sphere formation. We developed a method that demonstrates cultivated mesenchymal cells derived from dermis and lung tissue possess in vivo hair-inducing capacity via sphere formation.

Key words

Mesenchymal cells Sphere formation Skin-derived precursors (SKPs) 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive SurgeryKeio University, School of MedicineTokyoJapan

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