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Zebrafish pp 355–366Cite as

Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1451))

Abstract

Advances in genetic technologies enable the highly selective expression of transgenes in targeted neuronal cell types. Transgene expression can be used to noninvasively ablate, silence or activate neurons, providing a tool to probe their contribution to the control of behavior or physiology. Here, we describe the use of the tetrodotoxin (TTX)-resistant voltage-gated sodium channel Nav1.5 for either sensitizing neurons to depolarizing input, or isolating targeted neurons from surrounding neural activity, and methods for selective neuronal ablation using the bacterial nitroreductase NfsB.

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Acknowledgment

This work was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute for Child Health and Human Development.

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    Authors and Affiliations

    1. Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA

      Eric J. Horstick, Kathryn M. Tabor, Diana C. Jordan & Harold A. Burgess

    2. NIH, 6 Center Drive, Building 6B, Room 3B308, Bethesda, MD, 20892, USA

      Harold A. Burgess

    Authors
    1. Eric J. Horstick
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    2. Kathryn M. Tabor
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    3. Diana C. Jordan
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    4. Harold A. Burgess
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    Corresponding author

    Correspondence to Harold A. Burgess .

    Editor information

    Editors and Affiliations

    1. Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

      Koichi Kawakami

    2. Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

      E. Elizabeth Patton

    3. Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

      Michael Orger

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    Horstick, E.J., Tabor, K.M., Jordan, D.C., Burgess, H.A. (2016). Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels. In: Kawakami, K., Patton, E., Orger, M. (eds) Zebrafish. Methods in Molecular Biology, vol 1451. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3771-4_25

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    • DOI: https://doi.org/10.1007/978-1-4939-3771-4_25

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3769-1

    • Online ISBN: 978-1-4939-3771-4

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