Abstract
Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.
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Acknowledgments
This work was supported by the Kennedy Trust for Rheumatology Research. We would like to acknowledge the intellectual contributions of Dr. B. Gregory and the late Prof. F. Brennan to these studies. We thank Prof. M. Levings for pCCL and Prof. D. Trono for pMD2.G and psPAX vectors.
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Kennedy, A., Cribbs, A.P. (2016). Production and Concentration of Lentivirus for Transduction of Primary Human T Cells. In: Federico, M. (eds) Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools. Methods in Molecular Biology, vol 1448. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3753-0_7
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DOI: https://doi.org/10.1007/978-1-4939-3753-0_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3751-6
Online ISBN: 978-1-4939-3753-0
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