Abstract
Osteoclasts, specialized cells that degrade bone, are key components of the cellular system that regulates and maintains bone homeostasis. Aberrant function of osteoclasts can lead to pathological loss or gain of bone mass, such as in osteopetrosis, osteoporosis, and several types of cancer that metastasize to bone. Phosphorylation of osteoclast proteins on tyrosine residues is critical for formation of osteoclasts and for their proper function and responses to physiological signals. Here we describe preparation and growth of osteoclasts from bone marrow of mice, use of viral vectors to downregulate expression of endogenous proteins and to express exogenous proteins in osteoclasts, and analysis of signaling processes triggered by M-CSF, estrogen, and physical contact with matrix in these cells.
*The authors are contributed equally.
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Acknowledgements
This study was supported by grants from The Israel Science Foundation, from the Chief Scientist, Israel Ministry of Heath, and from the Kekst Family Institute for Medical Genetics and the David and Fella Shapell Family Center for Genetic Disorders Research, both of the Weizmann Institute of Science (to A.E.).
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Finkelshtein, E., Levy-Apter, E., Elson, A. (2016). Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling. In: Pulido, R. (eds) Protein Tyrosine Phosphatases. Methods in Molecular Biology, vol 1447. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3746-2_16
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DOI: https://doi.org/10.1007/978-1-4939-3746-2_16
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