In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

  • Rui Huang
  • Peter S. Conti
  • Kai ChenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1444)


Near-infrared fluorescence (NIRF) imaging is an emerging imaging technique for studying diseases at the molecular level. Optical imaging with a near-infrared emitting fluorophore for targeting tumor angiogenesis offers a noninvasive method for early tumor detection and efficient monitoring of tumor response to anti-angiogenesis therapy. CD13 receptor, a zinc-dependent membrane-bound ectopeptidase, plays important roles in regulating tumor angiogenesis and the growth of new blood vessels. In this chapter, we use CD13 receptor as an example to demonstrate how to construct CD13-specific NGR-containing peptides via bioorthogonal click chemistry for visualizing and quantifying the CD13 receptor expression in vivo by means of NIRF optical imaging.

Key words

Tumor angiogenesis Near-infrared fluorescence imaging Peptide-based probes Molecular imaging Cancer 



This work was supported by the American Cancer Society (#IRG-58-007-51), the Robert E. and May R. Wright Foundation, and the USC Department of Radiology.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Molecular Imaging Center, Department of Radiology, Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA

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