Fluorescence Lifetime Imaging of Cancer In Vivo

  • Owen Peng
  • Walter J. AkersEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1444)


Optical imaging of fluorescent reporters in animal models of cancer has become a common tool in oncologic research. Fluorescent reporters including fluorescent proteins, organic dyes, and inorganic photonic materials are used in fluorescence spectroscopy, microscopy, and whole body preclinical imaging. Fluorescence lifetime imaging provides additional, quantitative information beyond that of conventional fluorescence intensity signals, enabling signal multiplexing, background separation, and biological sensing unique to fluorescent materials.

Key words

Molecular imaging Microenvironment Drug delivery Oncology Animal model Time-domain Diffuse spectroscopy Optical Preclinical 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of RadiologyWashington University School of MedicineSt. LouisUSA

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