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Fluorescence Imaging of Inflammation in Live Animals

  • Mingzhou ZhouEmail author
  • Jie Cao
  • Walter J. Akers
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1444)

Abstract

Inflammation is associated with many diseases, such as stroke, cancer, and atherosclerosis. Noninvasive in vivo monitoring of inflammation can provide deeper understanding of such diseases, which might help to develop better treatment. Inflammation normally causes neutrophils and macrophages to generate reactive oxygen species (ROS) as the destruction tool, which can be used as a biomarker for inflammation. Near-infrared (NIR) window is optimal for in vivo fluorescence imaging owing to the reduced autofluorescence and low attenuation of light in biological tissues. Among NIR fluorescent probes, activatable probes have the promise of achieving high imaging contrast. In this chapter, we describe the method for in vivo fluorescence imaging of inflammation using a ROS-activatable NIR probe.

Key words

Inflammation ROS In vivo Near infrared fluorescence imaging Activatable probe 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of RadiologyWashington University School of MedicineSt. LouisUSA
  2. 2.School of Economics and ManagementThe Nanjing University of Information Science and TechnologyNanjingChina

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