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In Vivo Fluorescence Imaging pp 45–54Cite as

Fluorescence Imaging of Inflammation in Live Animals

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1444))

Abstract

Inflammation is associated with many diseases, such as stroke, cancer, and atherosclerosis. Noninvasive in vivo monitoring of inflammation can provide deeper understanding of such diseases, which might help to develop better treatment. Inflammation normally causes neutrophils and macrophages to generate reactive oxygen species (ROS) as the destruction tool, which can be used as a biomarker for inflammation. Near-infrared (NIR) window is optimal for in vivo fluorescence imaging owing to the reduced autofluorescence and low attenuation of light in biological tissues. Among NIR fluorescent probes, activatable probes have the promise of achieving high imaging contrast. In this chapter, we describe the method for in vivo fluorescence imaging of inflammation using a ROS-activatable NIR probe.

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Authors and Affiliations

  1. Department of Radiology, Washington University School of Medicine, Campus Box 8225, RM 3356, 4566 Scott Avenue, St. Louis, MO, 63110, USA

    Mingzhou Zhou & Walter J. Akers

  2. School of Economics and Management, The Nanjing University of Information Science and Technology, Nanjing, Jiangsu Province, China

    Jie Cao

Authors
  1. Mingzhou Zhou
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  2. Jie Cao
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  3. Walter J. Akers
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Corresponding author

Correspondence to Mingzhou Zhou .

Editor information

Editors and Affiliations

  1. Univ of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Mingfeng Bai

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© 2016 Springer Science+Business Media New York

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Zhou, M., Cao, J., Akers, W.J. (2016). Fluorescence Imaging of Inflammation in Live Animals. In: Bai, M. (eds) In Vivo Fluorescence Imaging. Methods in Molecular Biology, vol 1444. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3721-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3721-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3719-6

  • Online ISBN: 978-1-4939-3721-9

  • eBook Packages: Springer Protocols

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