FRET Imaging of Enzymatic Activities Using Smart Probes
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Tumor-related enzymes are extensively involved in the occurrence, development, invasion, and metastasis of tumors, indicating they hold the potential to serve as biomarkers for tumor diagnosis and treatment. Smart probes based on characteristic activities of these enzymes and fluorescence resonance energy transfer (FRET) have been widely developed for fluorescent imaging of enzymatic activities. Here, we describe the detailed chemical strategies for construction of smart probe and its application for FRET imaging of fibroblast activation protein alpha in vitro and in vivo.
Key wordsEnzyme Fluorescence resonance energy transfer Peptide Fluorescent imaging Smart probe
This work was supported in part by National Cancer Institute (NCI) 5R01 CA119053 (Z.C.) and the National Basic Research Program of China (No. 2011CB935800).
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