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In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes

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Book cover In Vivo Fluorescence Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1444))

Abstract

In vivo fluorescence imaging in the second near-infrared window (NIR-II window, 1000–1700 nm) is a powerful imaging technique that emerged in recent years. This imaging tool allows for noninvasive, deep-tissue visualization and interrogation of anatomical features and functions with improved imaging resolution and contrast at greater tissue penetration depths than traditional fluorescence imaging. Here, we present the detailed protocol for conducting NIR-II fluorescence imaging in live animals, including the procedures for preparation of biocompatible and NIR-II fluorescent carbon nanotube solution, live animal administration and NIR-II fluorescence image acquisition.

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Acknowledgments

We thank members from the Dai lab, in particular Shuo Diao, Alexander L. Antaris, and Dr. Xiaodong Zhang for helpful discussion and support. This study is supported by grants from the National Cancer Institute of US National Institute of Health to H.D. (5R01CA135109-02).

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA, 02138, USA

    Guosong Hong

  2. Department of Chemistry, Stanford University, Stanford, CA, 94305, USA

    Guosong Hong & Hongjie Dai

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  1. Guosong Hong
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  2. Hongjie Dai
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Correspondence to Guosong Hong or Hongjie Dai .

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Editors and Affiliations

  1. Univ of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Mingfeng Bai

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Hong, G., Dai, H. (2016). In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes. In: Bai, M. (eds) In Vivo Fluorescence Imaging. Methods in Molecular Biology, vol 1444. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3721-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3721-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3719-6

  • Online ISBN: 978-1-4939-3721-9

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