Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo
Imaging has become an indispensable tool in both clinical medicine and preclinical sciences. It enables doctors to locate sites of cancer/disease, track drug delivery, and guide operative planning, thus enhancing the treatment efficacy. Recently, we developed a multimodal theranostic lipid nanoparticles, named HPPS(NIR)-chol-siRNA with its built-in near-infrared (NIR) fluorescent probe core as a useful surrogate for tracking small interfering RNA (siRNA) delivery. By using the image co-registration of computed tomography (CT) and fluorescence molecular tomography (FMT), we achieved noninvasive assessment and treatment planning of siRNA delivery into the orthotopic tumor, thus enabling efficacious RNA interference (RNAi) therapy. In this chapter, we introduce this method to illustrate the use of CT-FMT co-registration for tracking drug delivery and guiding treatment planning in vivo.
Key wordsNanoparticles Fluorescence imaging Computed tomography Fluorescence molecular tomography HDL siRNA delivery
The authors thank funding support from the China–Canada Joint Health Research Initiative (NSFC-30911120489, CIHR CCI-102936), the Natural Sciences and Engineering Research Council of Canada, the Canadian Institutes for Health Research, the Ontario Institute for Cancer Research, the Prostate Cancer Canada, the DLVR Therapeutics, the Canada Foundation for Innovation, the Princess Margaret Cancer Foundation, and the Joey and Toby Tanenbaum/Brazilian Ball Chair in Prostate Cancer Research.
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