In Vivo Metal Ion Imaging Using Fluorescent Sensors

  • Genevieve C. Van de BittnerEmail author
  • Tasuku Hirayama
Part of the Methods in Molecular Biology book series (MIMB, volume 1444)


In vivo imaging in living animals provides the ability to monitor alterations of signaling molecules, ions, and other biological components during various life stages and in disease. The data gained from in vivo imaging can be used for biological discovery or to determine elements of disease progression and can inform the development and translation of therapeutics. Herein, we present theories behind small-molecule, fluorescent, metal ion sensors as well as the methods for their successful application to in vivo metal ion imaging, including ex vivo validation.

Key words

Fluorescence Near-infrared Copper In vivo imaging IVIS spectrum Wilson’s disease Metal Ion 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Genevieve C. Van de Bittner
    • 1
    Email author
  • Tasuku Hirayama
    • 2
  1. 1.Department of Radiology, Athinoula A. Martinos Center for Biomedical ImagingMassachusetts General Hospital, Harvard Medical SchoolCharlestownUSA
  2. 2.Laboratory of Medicinal & Pharmaceutical ChemistryGifu Pharmaceutical UniversityGifuJapan

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