In Vivo Cell Tracking Using Two-Photon Microscopy
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Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.
Key wordsFluorescent proteins Multiphoton microscopy Adipose tissue Lymph node Cell tracking Second harmonic generation Third harmonic generation
This work was supported by intramural funding of the Division of Intramural Research, National Heart, Lung, and Blood Institute, NIH. We thank Dr. Xingmin Feng (Hematology Branch, NHLBI) for providing the mice, Dr. Christian A. Combs for the overall support of NHLBI light microscopy core facility, and Brent Nettrour, Peter Pecoraro, for the technical support of the microscope.
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