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In Vivo Cell Tracking Using Two-Photon Microscopy

  • Daniela MalideEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 1444)

Abstract

Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.

Key words

Fluorescent proteins Multiphoton microscopy Adipose tissue Lymph node Cell tracking Second harmonic generation Third harmonic generation 

Notes

Acknowledgements

This work was supported by intramural funding of the Division of Intramural Research, National Heart, Lung, and Blood Institute, NIH. We thank Dr. Xingmin Feng (Hematology Branch, NHLBI) for providing the mice, Dr. Christian A. Combs for the overall support of NHLBI light microscopy core facility, and Brent Nettrour, Peter Pecoraro, for the technical support of the microscope.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Light Microscopy Core FacilityNational Heart, Lung, and Blood Institute, National Institute of HealthBethesdaUSA

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