Abstract
This chapter describes two procedures commonly used to examine airway function in mice. Airway function can be assessed in vivo using noninvasive or invasive methods. Noninvasive methods can be used to monitor respiratory function in mice without the involvement of restraint, anesthesia, or surgery. The methods allow for multiple animals to be monitored simultaneously and can be used in longitudinal studies requiring repeated measurements on the same animals. Invasive methods are used to assess airway function under anesthesia, in mechanically ventilated mice. Although used as terminal procedure, the invasive methods are most appropriate for direct assessment of lower airway dysfunction.
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References
Dakhama A, Lee YM, Gelfand EW (2005) Virus-induced airway dysfunction: pathogenesis and biomechanisms. Pediatr Infect Dis J 24(11 Suppl):S159–S169
Taube C, Dakhama A, Gelfand EW (2004) Insights into the pathogenesis of asthma utilizing murine models. Int Arch Allergy Immunol 135:173–186
Irvin CG, Bates JH (2003) Measuring the lung function in the mouse: the challenge of size. Respir Res 4:4
Hamelmann E, Schwarze J, Takeda K et al (1997) Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography. Am J Respir Crit Care Med 156:766–775
Tripp RA, Dakhama A, Jones LP et al (2003) The G glycoprotein of respiratory syncytial virus depresses respiratory rates through the CX3C motif and substance P. J Virol 77:6580–6584
Miyahara S, Miyahara N, Lucas JJ et al (2008) Contribution of allergen-specific and nonspecific nasal responses to early-phase and late-phase nasal responses. J Allergy Clin Immunol 121:718–724
Martin TR, Gerard NP, Galli SJ et al (1988) Pulmonary responses to bronchoconstrictor agonists in the mouse. J Appl Physiol 64:2318–2323
Levitt RC, Mitzner W (1988) Expression of airway hyperreactivity to acetylcholine as a simple autosomal recessive trait in mice. FASEB J 2:2605–2608
Takeda K, Hamelmann E, Joetham A, Shultz LD, Larsen GL, Irvin CG, Gelfand EW (1997) Development of eosinophilic airway inflammation and airway hyperresponsiveness in mast cell-deficient mice. J Exp Med 186(3):449–454
Lee YM, Miyahara N, Takeda K et al (2008) IFN-gamma production during initial infection determines the outcome of reinfection with respiratory syncytial virus. Am J Respir Crit Care Med 177:208–218
Sinnett EE, Jackson AC, Leith DE et al (2003) Fast integrated flow plethysmograph for small mammals. J Appl Physiol Respir Environ Exerc Physiol 50:1104–1110
O'Neil JJ, Raub JA (1984) Pulmonary function testing in small laboratory mammals. Environ Health Perspect 56:11–22
Delaunois A, Dedoncker P, Hanon E et al (2009) Repeated assessment of cardiovascular and respiratory functions using combined telemetry and whole-body plethysmography in the rat. J Pharmacol Toxicol Methods 60:117–129
Bates J, Irvin CG, Brusasco V et al (2004) The use and misuse of Penh in animal models of lung disease. Am J Respir Cell Mol Biol 31:373–374
Irvin CG, Tu YP, Sheller JR et al (1997) 5-Lipoxygenase products are necessary for ovalbumin-induced airway responsiveness in mice. Am J Physiol 272:L1053–L1058
Hantos Z, Daróczy B, Suki B et al (1992) Input impedance and peripheral inhomogeneity of dog lungs. J Appl Physiol 72:168–178
Bates JH, Irvin CG (2003) Measuring lung function in mice: the phenotyping uncertainty principle. J Appl Physiol 94:1297–1306
Acknowledgment
This work was supported by NIH grants R01 HD053557 and P01 ES018181 to AD.
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Dakhama, A., Gelfand, E.W. (2016). In Vivo Assessment of Airway Function in the Mouse Model. In: Tripp, R., Jorquera, P. (eds) Human Respiratory Syncytial Virus. Methods in Molecular Biology, vol 1442. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3687-8_16
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DOI: https://doi.org/10.1007/978-1-4939-3687-8_16
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