In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells

  • Srinivas S. Somanchi
  • Bridget A. Kennis
  • Vidya Gopalakrishnan
  • Dean A. Lee
  • James A. Bankson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1441)

Abstract

In order to assess the biodistribution, homing, and persistence of adoptively transferred natural killer (NK) cell immunotherapies, there is a need for imaging methodology suitable for use in preclinical studies with relevance to clinical translation. Amongst the available approaches, 19F-MRI is very appealing for in vivo imaging due to the absence of background signal, enabling clear detection of 19F labeled cells in vivo. Here we describe a methodology for in vivo imaging of adoptively transferred NK cells labeled with 19F nano-emulsion, using clinically translatable technology of 19F/1H magnetic resonance imaging.

Key words

NK cells Adoptive immunotherapy In vivo imaging 19Magnetic resonance imaging 

Notes

Acknowledgments

This work was supported in part by funding from the Addis Faith Foundation to VG, CURE Childhood Cancer funding to DAL, and by MD Anderson Cancer Center’s Core Grant (P30-CA016672).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Srinivas S. Somanchi
    • 1
  • Bridget A. Kennis
    • 1
  • Vidya Gopalakrishnan
    • 1
  • Dean A. Lee
    • 1
  • James A. Bankson
    • 2
  1. 1.Division of PediatricsThe University of Texas MD Anderson Cancer CenterHoustonUSA
  2. 2.Department of Imaging Physics, Division of Diagnostic ImagingThe University of Texas MD Anderson Cancer CenterHoustonUSA

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