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Natural Killer Cells

Volume 1441 of the series Methods in Molecular Biology pp 317-332

Date:

In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells

  • Srinivas S. SomanchiAffiliated withDivision of Pediatrics, The University of Texas MD Anderson Cancer Center
  • , Bridget A. KennisAffiliated withDivision of Pediatrics, The University of Texas MD Anderson Cancer Center
  • , Vidya GopalakrishnanAffiliated withDivision of Pediatrics, The University of Texas MD Anderson Cancer Center
  • , Dean A. LeeAffiliated withDivision of Pediatrics, The University of Texas MD Anderson Cancer Center
  • , James A. BanksonAffiliated withDepartment of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center Email author 

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Abstract

In order to assess the biodistribution, homing, and persistence of adoptively transferred natural killer (NK) cell immunotherapies, there is a need for imaging methodology suitable for use in preclinical studies with relevance to clinical translation. Amongst the available approaches, 19F-MRI is very appealing for in vivo imaging due to the absence of background signal, enabling clear detection of 19F labeled cells in vivo. Here we describe a methodology for in vivo imaging of adoptively transferred NK cells labeled with 19F nano-emulsion, using clinically translatable technology of 19F/1H magnetic resonance imaging.

Key words

NK cells Adoptive immunotherapy In vivo imaging 19F Magnetic resonance imaging