Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells

  • Alexander W. Kay
  • Dara M. Strauss-Albee
  • Catherine A. Blish
Part of the Methods in Molecular Biology book series (MIMB, volume 1441)

Abstract

Mass cytometry is a novel platform for high-dimensional phenotypic and functional analysis of single cells. This system uses elemental metal isotopes conjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracellular functional markers and surface phenotypic markers on natural killer cells.

Key words

CyTOF Mass cytometry Flow cytometry Natural killer cell Intracellular cytokine staining 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Alexander W. Kay
    • 1
  • Dara M. Strauss-Albee
    • 2
  • Catherine A. Blish
    • 2
    • 3
  1. 1.Department of PediatricsStanford University School of MedicineStanfordUSA
  2. 2.Stanford ImmunologyStanford University School of MedicineStanfordUSA
  3. 3.Department of MedicineStanford University School of MedicineStanfordUSA

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