Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli

  • Alessandra M. Martorana
  • Sara Motta
  • Paola Sperandeo
  • Pierluigi MauriEmail author
  • Alessandra PolissiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1440)


Cell envelope proteins in bacteria are typically difficult to characterize due to their low abundance, poor solubility, and the problematic isolation of pure surface fraction with only minimal contamination. Here we describe a method for cell membrane fractionation followed by mass spectrometry-based proteomics to analyze and determine protein abundance in bacterial membranes.

Key words

MudPIT Membrane proteins Gel-free Gram-negative cell envelope Outer membrane Cell fractionation 



We thank CNR Project “FaReBio di Qualità,” MIUR PRIN 2012WJSX8K, and Regione Lombardia Progetti di Ricerca Industriale e Sviluppo Sperimentale ID n. 30190679 for the financial support provided for this study. We thank Marta G. Bitonti for providing the MAProMA software.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Dipartimento di Biotecnologie e BioscienzeUniversità di Milano-BicoccaMilanItaly
  2. 2.Istituto di Tecnologie Biomediche, Consiglio Nazionale delle RicercheMilanItaly

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