Mycobacterium tuberculosis High-Throughput Screening

  • E. Lucile WhiteEmail author
  • Nichole A. Tower
  • Lynn Rasmussen
Part of the Methods in Molecular Biology book series (MIMB, volume 1439)


High-throughput screening is a valuable way to identify hit compounds that combined with a robust medicinal chemistry program could lead to the identification of new antibiotics. Here, we discuss our method for screening large compound libraries with virulent Mycobacterium tuberculosis, possibly one of the more difficult bacteria to use because of its slow growth and assignment to Biosafety Level-3 by the CDC and NIH. The principles illuminated here, however, are relevant to the execution of most bacteria high-throughput screens.

Key words

Mycobacterium tuberculosis Mtb H37Rv Tuberculosis TB High-throughput screening HTS Antitubercular Antibacterial Antimicrobial Antibiotics 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • E. Lucile White
    • 1
    Email author
  • Nichole A. Tower
    • 1
  • Lynn Rasmussen
    • 1
  1. 1.High Throughput Screening CenterSouthern Research InstituteBirminghamUSA

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