Visualization of HDAC9 Spatiotemporal Subcellular Localization in Primary Neuron Cultures

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1436)

Abstract

Histone deacetylase (HDAC) 9 is one of class IIa HDACs which are expressed in developing cortical neurons. The translocation of HDAC9 from the nucleus to the cytoplasm is induced by neuronal activity during postnatal development, and is involved in regulation of various gene expressions. Visualization of HDAC9 subcellular localization is a powerful tool for studying activity-dependent gene expression. Here, we describe a time-lapse imaging method using fluorescent protein-tagged HDAC9 in dissociated cortical neurons. This method reveals dynamic HDAC9-mediated gene expression in response to various signals.

Key words

HDAC9 cDNA cloning Primary cortical neuron cultures Time-lapse imaging Transfection Fluorescent imaging 

Notes

Acknowledgment

This work was supported by JSPS KAKENHI Grant Nos. 16700286 and 18300105 (N.S. & N.Y.) and by The Nakajima Foundation (N.S.).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Neuroscience Laboratories, Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan

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