Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation
Bacillus subtilis is the most commonly used Gram-positive bacterium to study cellular processes because of its genetic tractability. In addition, during nutrient limitation, B. subtilis undergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize B. subtilis during vegetative growth, and the developmental process of sporulation.
Key wordsBacillus subtilis Vegetative growth Sporulation Fluorescence microscopy Time-lapse microscopy
Support for this work comes from National Institutes of Health Grants GM086466 and GM073831 (to David Z. Rudner). X.W. was a long-term fellow of the Human Frontier Science Program. P.M.L. is a Helen Hay Whitney postdoctoral fellow.
- 9.Harwood CR, Cutting SM (1990) Molecular biological methods for Bacillus. Wiley, New York, NYGoogle Scholar