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Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis

  • Michelle HawkinsEmail author
  • John Atkinson
  • Peter McGlynn
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1431)

Abstract

Flow cytometry is a high-throughput technique that analyzes individual particles as they pass through a laser beam. These particles can be individual cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of Escherichia coli cells.

Key words

Flow cytometry Genome copy number Genome content Escherichia coli Replication Chromosome DnaA 

Notes

Acknowledgements

We thank Karen Hogg and the Imaging and Cytometry Laboratory in the Technology Facility at the University of York for technical assistance and support. This work was supported by BBSRC grant BB/I001859/1 and the University of York.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Michelle Hawkins
    • 1
    Email author
  • John Atkinson
    • 2
  • Peter McGlynn
    • 1
  1. 1.Department of BiologyUniversity of YorkYorkUK
  2. 2.School of Medical Sciences, Institute of Medical SciencesUniversity of AberdeenForesterhill, AberdeenUK

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