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Propagation of Chikungunya Virus Using Mosquito Cells

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore

    Swee Kim Ang, Shirley Lam & Justin Jang Hann Chu

Authors
  1. Swee Kim Ang
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  2. Shirley Lam
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  3. Justin Jang Hann Chu
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Corresponding author

Correspondence to Justin Jang Hann Chu .

Editor information

Editors and Affiliations

  1. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Swee Kim Ang

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© 2016 Springer Science+Business Media New York

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Ang, S.K., Lam, S., Chu, J.J.H. (2016). Propagation of Chikungunya Virus Using Mosquito Cells. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

  • eBook Packages: Springer Protocols

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