Abstract
Synthetic peptide-based diagnosis of Chikungunya can be an efficient and more accessible approach in immunodiagnostics. Here, we describe the identification of Chikungunya-specific 40 kD protein for development of synthetic peptide-based enzyme-linked immunosorbent assay for the detection of Chikungunya virus-specific antibodies in the patient’s sample. The total sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profile of the patient’s sample can be done to identify specific protein bands. The identified proteins can be subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for characterization. After characterization, immunogenic peptides can be designed using softwares and subsequently synthesized chemically. The peptides can be used to develop more specific, sensitive, and simpler diagnostic assay.
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Kashyap, R.S., Bhullar, S.S., Chandak, N.H., Taori, G.M. (2016). Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_4
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DOI: https://doi.org/10.1007/978-1-4939-3618-2_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3616-8
Online ISBN: 978-1-4939-3618-2
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