Chikungunya Virus pp 273-282 | Cite as
Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
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Abstract
Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by counting and comparing number of plaques (localized area of infection due to cytopathic effect) with a standard amount of virus. Conventional neutralizing test uses plaque-reduction neutralization test (PRNT) to determine neutralizing antibody titers against Chikungunya virus (CHIKV). Here we describe the plaque reduction neutralization assay (PRNT) using Vero cell lines to obtain neutralizing antibody titers.
Key words
Plaque reduction neutralization test Antibody Chikungunya Neutralizing antibody Neutralizing assay Vero cell linesNotes
Acknowledgements
We would like to acknowledge Dr. Chang-Kweng Lim, Department of Virology I, National Institute of Infectious Diseases, Japan, for generously providing us with a figure on CHIKV plaques (Fig. 3). This work was supported in part by the research grant, Research on Emerging and Re-emerging Infectious Diseases (H26-shinkou-jitsuyouka-007), from the Ministry of Health, Labour and Welfare, Japan, the Environment Research and Technology Development Fund (S-8) of the Ministry of the Environment, and a Grant-in-Aid for Young Scientists (B) from JSPS (26870872).
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