Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin

  • Camille Malosse
  • Sandrine HenriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)


The improvement of dendritic cell subset isolation from tissues and the use of appropriate surface markers allowed to decipher their heterogeneity but also allowed to unravel some specific functions that are valuable for vaccine design as well as for a better understanding of the in situ pathophysiology upon infection. Here, we describe the procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.

Key words

Skin Epidermis Dermis Dispase Collagenase 4 Percoll Fc Block Monoclonal antibodies Flow cytometry 



This work is supported by ANR JCJC Skin DCs.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Centre d’Immunologie de Marseille-Luminy (CIML)UM2 Aix-Marseille UniversitéMarseille Cedex 9France
  2. 2.INSERM U1104Marseille Cedex 9France
  3. 3.CNRS UMR7280Marseille Cedex 9France

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