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Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

  • Charlotte L. ScottEmail author
  • Pamela B. WrightEmail author
  • Simon W. F. Milling
  • Allan McI Mowat
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Abstract

The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.

Key words

Dendritic cells Subsets Isolation Identification Intestine 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life SciencesUniversity of GlasgowScotlandUK
  2. 2.Laboratory of Mucosal Immunology and Immunoregulation, Inflammation Research Centre (IRC)VIB Ghent UniversityGhent (Zwijnaarde)Belgium
  3. 3.Department of Biomedical Molecular BiologyGhent University HospitalGhentBelgium

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