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In Vivo Imaging of Cutaneous DCs in Mice

  • Gyohei Egawa
  • Kenji KabashimaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Abstract

Varieties of cells orchestrate immune responses. To capture such dynamic phenomena, intravital imaging is an important technique, and it may provide substantial information that is not available using conventional histological analyses. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of biological samples with high spatiotemporal resolution, and it has now become the leading method for in vivo imaging studies. Here we describe a basic method for in vivo imaging of dendritic cells (DCs) in the mouse ear skin using multiphoton microscopy.

Key words

In vivo imaging Multiphoton microscopy Dendritic cells 

Notes

Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of DermatologyKyoto University Graduate School of MedicineKyotoJapan
  2. 2.PRESTO, Japan science and Technology AgencySaitamaJapan
  3. 3.Singapore Immunology Network (SIgN) and Institute of MedicalBiology, Agency for Science, Technology and Research (A*STAR)BiopolisSingapore

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