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In Vivo Ablation of a Dendritic Cell Subset Expressing the Chemokine Receptor XCR1

  • Hiroaki Hemmi
  • Katsuaki Hoshino
  • Tsuneyasu KaishoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Abstract

Dendritic cells (DCs) are one of the key populations controlling immune responses. To establish a cell depletion system in vivo, human diphtheria toxin (DT) receptor (DTR) is transduced to the mice in which DTR is expressed under the control of a specific promoter. In these mice, DTR-expressing cells are inducibly depleted after DT injection. Using this system, analysis of mouse models in which DTR was expressed under the CD11c promoter has contributed to our knowledge of DC biology by depleting CD11c+ cells. Other mouse models to inducibly eliminate specific DC subsets upon DT treatment have been also generated. Here, we describe a new mouse model in which the XCR1+ DC subset is inducibly and transiently depleted in vivo.

Keyword

Dendritic cells Dendritic cell subset Inducible ablation of dendritic cell subsets Diphtheria toxin Diphtheria toxin receptor 

Notes

Acknowledgments

This work was supported by the Kishimoto Foundation; Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science; Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology; and the Uehara Memorial Foundation.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Hiroaki Hemmi
    • 1
    • 2
    • 3
  • Katsuaki Hoshino
    • 1
    • 2
    • 4
  • Tsuneyasu Kaisho
    • 1
    • 2
    • 3
    Email author
  1. 1.Laboratory for Immune Regulation, WPI Immunology Frontier Research CenterOsaka UniversitySuitaJapan
  2. 2.Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical SciencesYokohamaJapan
  3. 3.Department of Immunology, Institute of Advanced MedicineWakayama Medical UniversityWakayamaJapan
  4. 4.Department of Immunology, Faculty of MedicineKagawa UniversityKita-gunJapan

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