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Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues

  • Naomi McGovern
  • Andreas Schlitzer
  • Baptiste Janela
  • Florent GinhouxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Abstract

The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.

Key words

Human tissue processing Antigen-presenting cells Flow cytometry Dendritic cells 

Notes

Acknowledgment

This work was supported by core grants of the Singapore Immunology Network.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Naomi McGovern
    • 1
  • Andreas Schlitzer
    • 1
  • Baptiste Janela
    • 1
  • Florent Ginhoux
    • 1
    Email author
  1. 1.Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR)SingaporeSingapore

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