Gastrointestinal Physiology and Diseases pp 189-196 | Cite as
Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells
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Abstract
Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host–bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.
Key words
Group 3 Innate lymphoid cells Intestine Purification Rag1 Adoptive transferNotes
Acknowledgement
This work was supported by the National Natural Science Foundation of China (31570923 to X.G.) and National Institutes of Health grant (DK100427-01A1 to Y.X.F.).
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