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Electrochemical Detection of Nitric Oxide in Plant Cell Suspensions

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1424)

Abstract

Nitric oxide is a hydrophobic radical acting as a physiological mediator in plants. Because of its unique properties, the detection of NO in plant tissues and cell suspensions remains a challenge. For this purpose, several techniques are used, each having certain advantages and limitations such as interferences with other species, questionable sensitivity, and/or selectivity or ex situ measurement. Here we describe a very attractive approach for tracking NO in plant cell suspensions using a NO-sensitive homemade platinum/iridium-based electrochemical microsensor. This method constitutes the absolute real-time proof of the production of free NO in physiological conditions.

Key words

Nitric oxide Plant cell suspensions Electrochemical detection Homemade electrode 

Notes

Acknowledgement

This work was supported by La Région de Bourgogne PARI AGRALE 8 project, the University of Burgundy Bonus Qualité Recherche project, and the ANR PIANO (A.B-B. and D.W.).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Unité de Technologies Chimiques et Biologiques pour la Santé, Chimie ParisTechPSL Research UniversityParisFrance
  2. 2.Unité de Technologies Chimiques et Biologiques pour la Santé UMR 8258CNRSParisFrance
  3. 3.Unité de Technologies Chimiques et Biologiques pour la SantéUniversité Paris DescartesParisFrance
  4. 4.Unité de Technologies Chimiques et Biologiques pour la Santé (N°1022)INSERMParisFrance
  5. 5.Université de Bourgogne, UMR 1347 AgroécologieDijonFrance
  6. 6.ERL CNRS 6300DijonFrance

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