Abstract
Recently mRNA profiling has been widely proposed as a universal tool for biological fluids identification. Here, we describe a test for vaginal fluid identification that combines detection of five markers: vaginal mRNAs and Lactobacilli in end-point PCR reaction. The test detects the following transcripts: HBD1 (Human beta-defensin 1), MUC4 (Mucin 4), MMP11 (Matrix metalloproteinase 11), housekeeping gene G6PDH (glucose 6-phosphate dehydrogenase), and the 16S–23S rRNA intergenic spacer regions of L. crispatus and L. gasseri/L. johnsonii. Simultaneous analysis of five vaginal markers and a housekeeping gene ensures high specificity and reliability in the detection of vaginal material, which could not be obtained using detection of a single marker.
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Acknowledgments
This research was co-financed by the European Union through the European Social Fund within the framework of the Human Capital Operational Programme.
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Jakubowska, J., Maciejewska, A., Pawłowski, R. (2016). mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification. In: Goodwin, W. (eds) Forensic DNA Typing Protocols. Methods in Molecular Biology, vol 1420. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3597-0_3
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DOI: https://doi.org/10.1007/978-1-4939-3597-0_3
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