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A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells

Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Apoptosis, or programmed cell death, is a tightly regulated cellular event that plays an important role in both normal developmental processes and many pathological states. The induction of apoptosis is tightly regulated through the coordinated action of members of the caspase family of proteases. Here we discuss a relatively inexpensive protocol for monitoring the induction and progression of apoptosis using a genetically encoded fluorescence resonance energy transfer (FRET)-based biosensor of the executioner caspase, caspase-3, in living suspension cells.

Key words

Genetically encoded biosensor Apoptosis Fluorescence resonance energy transfer Caspase 3 Sensor for activated caspases based on FRET (SCAT3) Diepoxybutane 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Energy and Environmental StudiesNorth Carolina A&T State UniversityGreensboroUSA
  2. 2.Department of BiologyNorth Carolina A&T State UniversityGreensboroUSA

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