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Detection of Apoptosis: From Bench Side to Clinical Practice

Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Apoptosis or programmed cell death is implicated in several pathological conditions, such as cancer and neurodegenerative diseases. An increasing number of therapies are developed by targeting apoptosis signaling components to either induce or inhibit apoptosis in target cells. For these reasons, it is critical to develop appropriate analytical methods for the detection of apoptotic cell death in the context of monitoring relevant disease progression and therapeutic effects of clinical treatments (e.g., chemotherapy in cancer patients). This review provides an overview of the currently used methods for detection of apoptosis and their applications in research and clinical practice.

Key words

Apoptosis Apoptosis detection In vitro apoptosis detection In vivo apoptosis detection Clinical apoptosis detection DNA fragmentation TUNEL Caspase activation detection Phosphatidylserine externalization 

Notes

Disclaimer

This chapter reflects the views of the author and should not be constructed to represent FDA’s views or policies.

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© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Office of Biotechnology Products, Center for Drug Evaluation and ResearchFood and Drug AdministrationSilver SpringUSA

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