Novel Electrochemical Biosensor for Apoptosis Evaluation
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Apoptosis evaluation is one of the most important tasks of toxicology. By using a peptide as the recognition element, and assembling apoptotic cells on a solid surface, we have established a novel electrochemical method for the detection of apoptosis levels. Such a peptide-based electrochemical biosensor is simple, cost-effective, convenient, and sensitive. Since the results obtained are well in line with other standard methods, this method holds a great potential towards the analysis of apoptosis and its applications. In this chapter, we introduce a general overview of this technical approach for detecting apoptotic cells. We discuss its advantages over the ordinary methods. We also provide practical guidelines for designing studies, and summarize the step-by-step protocols used in our lab for sample preparation, electrode modification, and accurate electrochemical quantification of apoptotic cells.
Key wordsAnalytical chemistry Apoptosis Electrochemical analysis Phosphatidylserine Peptide Differential pulse voltammetry Electrochemical impedance spectroscopy Cyclic voltammetry
This work was supported by the National Natural Science Foundation of China (Grant No. 21307154 and 31400847) and the National Key Instrument Developing Project of China (Grant No. ZDYZ2013-1).
Differential pulse voltammetry
Electrochemical impedance spectroscopy
Phosphate buffered saline
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