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Programmed Cell Death pp 213–229Cite as

Preparing Samples for Crystallization of Bcl-2 Family Complexes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1419))

Abstract

High-resolution protein structures determined by X-ray crystallography or NMR have proven invaluable for deciphering the molecular mechanisms underlying the function of a vast range of proteins. Here, we describe methods to generate complexes of proteins belonging to the Bcl-2 family of proteins with either biological ligands or small molecule antagonists.

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Acknowledgements

This work was supported by an Australian Research Council Future Fellowship (FT130101349) to MK and a National Health and Medical Research Council Senior Research Fellowship to PEC as well as project grants 1079706, 1059331, and 1023055 (to PEC) and 1082383 and 1007918 (to MK). We also thank Amanda Voudouris for assistance in preparing the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Physical Sciences 4, Room 205, Kingsbury Drive, Bundoora, Melbourne, VIC, 3086, Australia

    Marc Kvansakul

  2. La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia

    Marc Kvansakul

  3. The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia

    Peter E. Czabotar

  4. Department of Medical Biology, The University of Melbourne, Melbourne, VIC, 3052, Australia

    Peter E. Czabotar

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  1. Marc Kvansakul
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  2. Peter E. Czabotar
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Correspondence to Peter E. Czabotar .

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Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Inst for Mol Sci, La Trobe Univ, Bundoora, Victoria, Australia

    Hamsa Puthalakath

  2. Department of Biochemistry and Genetics, La Trobe Inst for Mol Sci, La Trobe Univ, Bundoora, Victoria, Australia

    Christine J. Hawkins

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Kvansakul, M., Czabotar, P.E. (2016). Preparing Samples for Crystallization of Bcl-2 Family Complexes. In: Puthalakath, H., Hawkins, C. (eds) Programmed Cell Death. Methods in Molecular Biology, vol 1419. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3581-9_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3581-9_16

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