Abstract
Naturally occurring proteins often consist of multiple distinct domains joined by linker regions. Similarly, the ability to combine globular protein domains through engineered linkers would allow the creation of a wide variety of complex and useful multifunctional proteins. Recent advances in computational design of protein structures have enabled highly accurate design of novel protein structures. In this chapter we outline a computational protocol for the de novo design of protein linkers, and apply this protocol to the design of a helical linker between two rigid protein domains.
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© 2016 Springer Science+Business Media New York
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Kuhlman, B., Jacobs, T., Linskey, T. (2016). Computational Design of Protein Linkers. In: Stoddard, B. (eds) Computational Design of Ligand Binding Proteins. Methods in Molecular Biology, vol 1414. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3569-7_20
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DOI: https://doi.org/10.1007/978-1-4939-3569-7_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3567-3
Online ISBN: 978-1-4939-3569-7
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